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electrocompetent megax dh10b e coli bacteria  (Thermo Fisher)


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    Thermo Fisher electrocompetent megax dh10b e coli bacteria
    Electrocompetent Megax Dh10b E Coli Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrocompetent+megax+dh10b+e+coli+bacteria/med_rxiv__2024__04__13__24305761-294-18-25
    Average 86 stars, based on 1 article reviews
    electrocompetent megax dh10b e coli bacteria - by Bioz Stars, 2026-09
    86/100 stars

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    Chromatin Immunoprecipitation:

    Article Title: A novel functional genomics atlas coupled with convolutional neural networks facilitates clinical interpretation of disease relevant variants in non-coding regulatory elements
    Article Snippet: Briefly, 5 μL of purified adaptor ligated DNA for each replicate were separately amplified with primers 147 STARRseq libr FW and 148 STARRseq libr RV ( Arnold et al ., 2013 ), and used in a Gibson assembly reaction with an AgeI-SalI digested STARR-seq plasmid and a home-made Gibson reaction buffer (100mM Tris-HCl, 10mM MgCl2, 0.2 mM dNTP, 0.5U Phusion DNA polymerase (NEB), 0.16U 50 T5 exonuclease (NEB)). .. Gibson reactions for the ChIP replicates were pooled together, purified by ethanol precipitation and used for electroporation into electrocompetent MegaX DH10b E. coli bacteria (C640003, Invitrogen), according to manufacturer’s instructions. ..

    Purification:

    Article Title: A novel functional genomics atlas coupled with convolutional neural networks facilitates clinical interpretation of disease relevant variants in non-coding regulatory elements
    Article Snippet: Briefly, 5 μL of purified adaptor ligated DNA for each replicate were separately amplified with primers 147 STARRseq libr FW and 148 STARRseq libr RV ( Arnold et al ., 2013 ), and used in a Gibson assembly reaction with an AgeI-SalI digested STARR-seq plasmid and a home-made Gibson reaction buffer (100mM Tris-HCl, 10mM MgCl2, 0.2 mM dNTP, 0.5U Phusion DNA polymerase (NEB), 0.16U 50 T5 exonuclease (NEB)). .. Gibson reactions for the ChIP replicates were pooled together, purified by ethanol precipitation and used for electroporation into electrocompetent MegaX DH10b E. coli bacteria (C640003, Invitrogen), according to manufacturer’s instructions. ..

    Ethanol Precipitation:

    Article Title: A novel functional genomics atlas coupled with convolutional neural networks facilitates clinical interpretation of disease relevant variants in non-coding regulatory elements
    Article Snippet: Briefly, 5 μL of purified adaptor ligated DNA for each replicate were separately amplified with primers 147 STARRseq libr FW and 148 STARRseq libr RV ( Arnold et al ., 2013 ), and used in a Gibson assembly reaction with an AgeI-SalI digested STARR-seq plasmid and a home-made Gibson reaction buffer (100mM Tris-HCl, 10mM MgCl2, 0.2 mM dNTP, 0.5U Phusion DNA polymerase (NEB), 0.16U 50 T5 exonuclease (NEB)). .. Gibson reactions for the ChIP replicates were pooled together, purified by ethanol precipitation and used for electroporation into electrocompetent MegaX DH10b E. coli bacteria (C640003, Invitrogen), according to manufacturer’s instructions. ..

    Electroporation:

    Article Title: A novel functional genomics atlas coupled with convolutional neural networks facilitates clinical interpretation of disease relevant variants in non-coding regulatory elements
    Article Snippet: Briefly, 5 μL of purified adaptor ligated DNA for each replicate were separately amplified with primers 147 STARRseq libr FW and 148 STARRseq libr RV ( Arnold et al ., 2013 ), and used in a Gibson assembly reaction with an AgeI-SalI digested STARR-seq plasmid and a home-made Gibson reaction buffer (100mM Tris-HCl, 10mM MgCl2, 0.2 mM dNTP, 0.5U Phusion DNA polymerase (NEB), 0.16U 50 T5 exonuclease (NEB)). .. Gibson reactions for the ChIP replicates were pooled together, purified by ethanol precipitation and used for electroporation into electrocompetent MegaX DH10b E. coli bacteria (C640003, Invitrogen), according to manufacturer’s instructions. ..

    Bacteria:

    Article Title: A novel functional genomics atlas coupled with convolutional neural networks facilitates clinical interpretation of disease relevant variants in non-coding regulatory elements
    Article Snippet: Briefly, 5 μL of purified adaptor ligated DNA for each replicate were separately amplified with primers 147 STARRseq libr FW and 148 STARRseq libr RV ( Arnold et al ., 2013 ), and used in a Gibson assembly reaction with an AgeI-SalI digested STARR-seq plasmid and a home-made Gibson reaction buffer (100mM Tris-HCl, 10mM MgCl2, 0.2 mM dNTP, 0.5U Phusion DNA polymerase (NEB), 0.16U 50 T5 exonuclease (NEB)). .. Gibson reactions for the ChIP replicates were pooled together, purified by ethanol precipitation and used for electroporation into electrocompetent MegaX DH10b E. coli bacteria (C640003, Invitrogen), according to manufacturer’s instructions. ..



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    86
    Thermo Fisher electrocompetent megax dh10b e coli bacteria
    Electrocompetent Megax Dh10b E Coli Bacteria, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/electrocompetent+megax+dh10b+e+coli+bacteria/med_rxiv__2024__04__13__24305761-294-18-25
    Average 86 stars, based on 1 article reviews
    electrocompetent megax dh10b e coli bacteria - by Bioz Stars, 2026-09
    86/100 stars
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